Medicago truncatula Handbook

ISBN 0-9754303-1-9
Preface to the Medicago truncatula Handbook

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Biology of Medicago truncatula

Morphology, development and plant architecture of M. truncatula: Vegetative development (D. Moreau)

Evolutionary and Ecological Genetics of Medicago truncatula (T. Bataillon, J. Ronfort)

Seed Biology of Medicago truncatula (K. Gallardo, C. Lesignor, M. Darmency, J. Burstin, R. Thompson, C. Rochat, J.-P. Boutin, H. Kuester, J. Buitink, O. Leprince, A. Limami, M.A. Grusak)

Response of Medicago truncatula to abiotic stress (A.M. Limami, C. Ricoult, E. Planchet, E.M. González, R. Ladrera, E. Larrainzar, C.Arrese-Igor, F. Merchan, M. Crespi, F. Frugier, K. Avia, I. Lejeune-Hénaut)

Symbiotic interactions: • Arbuscular mycorrhiza (V. Gianinazzi-Pearson, I. Maldonado-Mendoza, M. Lopez-Meyer, S. Weidmann and M. J. Harrison)

Pathogenic interactions: • Fungi (S. Ellwood, J. Lichtenzveig, T. Pfaff, L. Kamphuis, R. Oliver)
• Medicago truncatula as a model for studying interactions between root parasitic plants and legumes (A. Pérez-de-Luque, M.D. Lozano, A.M. Maldonado, J.V. Jorrín, M.A. Dita, J. Die, B. Román, D. Rubiales)

M. truncatula resources

M. truncatula stock centre (R. Nair , S.J. Hughes, S. Ellwood, R. Oliver, S.L. Green, M. Delalande, J. Wen, G. Oldroyd)

Wild accessions/populations (S. Greene, S.J. Hughes, R. Nair, T. Huguet, M.E. Aouani, J.M. Prosperi, M. Delalande)

Medicago truncatula cultivars (R. Nair, J. Howie, M. Delalande)

Bionformatics resources for Medicago truncatula - 2006: • UrMeLDB: The European Medicago genome database (K. Mayer et al.)
• Medicagoo: Searching Medicago resources (J. Gouzy et al.)
• EST analysis and expression profiling software from Bielefeld University (H. Küster et al.)
• The Legume Information System (LIS): An integrated, dynamic comparative legume information resource (G.D. May et al.)
• MtDB and medicago.org (E. Retzel, et al.)
• The Medicago truncatula Genome Sequencing Website (N.D. Young, et al.)

Medicago truncatula cDNA and genomic libraries (P. Gamas, F. Debellé, H. Berges, L. Godiard, A. Niebel, E.P. Journet, J. Gouzy)

Mutant gene nomenclature in Medicago truncatula (J. Frugoli)

M. truncatula protocol chapters

Handling Medicago truncatula as a whole plant:
growth, propagation, crossings, as well as abiotic stress and interactions with other organisms

Overview (E-P Journet)

Seed storage and germination (J. Garcia, D.G. Barker and E-P Journet)

Growing M. truncatula: choice of substrates and growth conditions (D.G. Barker, T. Pfaff, D. Moreau, E. Groves, S. Ruffel, M. Lepetit, S. Whitehand, F. Maillet, R. M. Nair, E-P Journet)

Vernalization, crossings and testing for pollen viability (M. Chabaud, J. Lichtenzveig, S. Ellwood, T. Pfaff, E-P Journet)

Phenotyping (D. Moreau, V. Bourion, S. Fournier, H. De Larambergue, G. Duc, C. Salon, A.S.Voisin, C. Jeudy)

Cuttings and grafts (S. Cros-Arteil, T. Pfaff, D.G. Barker and E-P Journet)

Rhizobial inoculation and nodulation of Medicago truncatula (E-P Journet, F. de Carvalho-Niebel, A. Andriankaja, T. Huguet, D.G. Barker)

Inoculation and growth with mycorrhizal fungi (M. Chabaud, M. Harrison, F. de Carvalho-Niebel, G. Bécard, D G. Barker)

Inoculation and growth with soil borne pathogenic fungi (J. Lichtenzveig, J. Anderson, G. Thomas, R. Oliver, K. Singh)

Inoculation and growth with foliar pathogenic fungi (S. Ellwood, L. Kamphuis, T. Pfaff, R. Oliver, D. Samac, D. Foster-Hartnett, B. Tivoli, C. Onfroy, A. Moussart, Villegas A.M., Sillero J.C., Rubiales D.)

Inoculation and growth with root parasitic weeds (M. Fernández-Aparicio, A. Pérez-de-Luque, M.D. Lozano, D. Rubiales)

Medicago truncatula resistance to Oomycetes (A. Moussart, B. Tivoli, D. Samac, N. D'Souza)

Medicago truncatula interaction with insects (O. Edwards, J. Klingler, L. Gao, K. Korth, K. Singh)

Cytology and microscopy

Immunolocalization (B. Hause, F. Frugier, M. Crespi)

Flow cytometry (ploidy determination, cell cycle analysis, DNA content per nucleus) (S.J. Ochatt)

Transformation and tissue culture

Cell suspension cultures (A. Iantcheva, M. Vlahova, A. Atanassov, A. S. Duque, S. Araújo, D. F. dos Santos, P. Fevereiro)

Agrobacterium rhizogenes-mediated root transformation (M. Chabaud, A. Boisson-Dernier, J. Zhang, C.G. Taylor, O. Yu, D.G. Barker)

Agrobacterium tumefaciens-mediated transformation and in vitro plant regeneration of M. truncatula (M. Chabaud, P. Ratet, S. de Sousa Araújo, A.S. Roldão Lopes Amaral Duque, M. Harrison, D.G. Barker)

Somatic embryogenesis from leaf explants (A. Iantcheva, M. Vlahova, A. Atanassov)

Genetic mapping

Comparative mapping (A. Seres, G. Deák, G. Tóth, G. Aubert, J. Burstin, N. Ellis, G. B. Kiss)

Tools for gene identification, silencing and knockout and mutant screening

Mutant generation using gamma rays (G. Duc, A. Pelletier)

Insertional mutagenesis in M. truncatula using Tnt1 retrotransposon (P. Ratet, A. Porcedu, M. Tadege, K.S. Mysore)

Mutant screening / phenology key (D. Moreau and N. Munier-Jolain)

Sharing phenotypic data: A coding system and a developmental model (J. Hanan, B. Bucciarelli, C.P. Vance)

Functional genomics

Transcriptomics (H. Küster, A. Becker, D. Samac, M. Tesfaye)

Proteomics (S. Nagaraj, Z. Lei, B. Watson, L. Sumner, K. Gallardo, E. Dumas-Gaudot, G. Recorbet, F. Robert, O. Thiery, B. Valot, U. Mathesius, E. Triplett)